University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, March 28th 2019
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Jerome C. Mertz, College of Engineering, Boston University
Subject: Fast, volumetric imaging with microscopes

Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. Camera-based microscopes are typically hampered by the problem of out-of-focus background which undermines image contrast. This background must be reduced, or eliminated, to achieve volumetric imaging. Alternatively, scanning techniques such as confocal and multiphoton microscopy can provide high contrast and high speed, but their generalization to volumetric imaging requires an axial scanning mechanism, which, in general, drastically reduces speed. I will describe a variety of strategies we have developed to enable fast, high-contrast, volumetric imaging over large length scales. These strategies include targeted-illumination widefield microscopy, multi-z confocal microscopy and reverberation multiphoton microscopy. I will discuss the principles of these strategies and present experimental validations.

Faculty Host: Jochen Mueller

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