Biophysics Seminar

Week of Monday, September 24th 2018

Thursday, October 4th 2018
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Jon Garamella, graduate student in Vincent Noireaux’s lab, School of Physics and Astronomy, University of Minnesota
Subject: Synthetic cell prototyping using a cell-free transcription-translation system (TXTL)

Cell-free Transcription-translation (TXTL) systems can be used in synthetic cell engineering to reconstitute cellular processes and functions. The bottom-up construction of cell-sized compartments programmed with DNA that are capable of sensing their chemical and physical environment remains challenging. Here, we develop techniques to prototype synthetic cells with passive and active membrane functionality. We construct liposomes programmed to intake nutrients from the surroundings and respond to external, mechanical stimuli using alpha-hemolysin and the mechanosensitive channel of large conductance, respectively. High-throughput techniques to test the viability of membrane proteins are also described.

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