University of Minnesota
School of Physics & Astronomy
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Jolene Johnson

Research Assistant

S61 Tate, 624-6045, email jjohnson @ physics.umn.edu

Doctoral Dissertation Fellow, University of Minnesota-TC, 2011-2012
Adjunct Professor, University of St. Thomas, 2011
Graduate Research Asssitant, 2007-2011
Women in Physics and Astronomy Coordinator, 2008-2009
Teaching Assistant, 2005-2008

Current Research

Protein-protein interactions are fundamental to all cellular processes. Fluorescence fluctuation spectroscopy (FFS) is a technique with the unique capability of quantifying these protein interactions directly inside the living cell. Previously FFS has been used to study the interaction of small protein complexes, however there are many systems that involve larger complexes, such as viral assembly and intracellular vesicle trafficking. We have developed microfluidic chips that in combination with FFS brightness analysis have greatly expanded the range of biological samples accessible by FFS. We have applied these techniques to study the viruses HIV-1, HTLV-1 and the vesicle protein PI4KII beta kinase.

Selected Publications

Keir H. Fogarty, Yan Chen, Iwen F. Grigsby, Patrick J. Macdonald, Elizabeth Smith, Jolene L. Johnson, Jonathan Rawson, Louis M. Mansky, and Joachim D. Mueller, Characterization of Cytoplasmic Gag-Gag Interactions by Dual-Color z-scan Fluorescence Fluctuation Spectroscopy, Biophysical Journal [abstract]

Jolene L. Johnson, Yan Chen, Joachim D. Mueller, Characterization of Brightness and Stoichiometry of Bright Particles by Flow-Fluorescence Fluctuation Spectroscopy, Biophysical Journal [abstract]

Iwen F. Grigsby , Wei Zhang, Jolene L. Johnson, Keir H. Fogarty, Yan Chen, Jonathan M. Rawson, Aaron J. Crosby, Joachim D. Mueller, Louis M. Mansky, Biophysical analysis of HTLV-1 Particles Reveals Novel Insights into Particle Morphology and Gag Stoichiometry, Retrovirology [abstract]

Yan Chen, Jolene L. Johnson, Patrick J. Macdonald, Bin Wu, and Joachim D. Mueller, Chapter 16 - Observing Protein Interactions and Their Stoichiometry in Living Cells by Brightness Analysis of Fluorescence Fluctuation Experiments, Methods of Enzymology [abstract]

Education

MS Physics, 2008, University of Minnesota-TC
BA Physics & Music, 2005, Gustavus Adolphus College