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Tentative Schedule for the Fluorescence Workshop
Lecture room: 157 Tate Laboratory of Physics
Lab room: S60 Tate Laboratory of Physics
Lab room: S60 Tate Laboratory of Physics
Day 1 (June 8th)
| Lectures: | 8:30 � 8:45 | Introduction |
| 8:45 -9:45 | Basic Spectroscopic Principles | |
| 9:45 -10:45 | Fluorescence Principles | |
| 10:45 - 12:00 | Quantum Yield + Polarization (1) | |
| Lunch Break: | 12:00 -13:15 | |
| Lab: | 13:15-17:00 | Introduction to the fluorometer, excitation and emission spectra, polarization |
Day 2 (June 9th)
| Lectures: | 9:00 -10:30 | Fluorescence Lifetime + Polarization (2) |
| 10:30 -11:30 | Quenching + FRET | |
| 11:30 � 12:00 | Fluorescence Essays | |
| Lunch Break: | 12:00 -13:15 | |
| Lab: | 13:15 - 17:00 | Characterize antibody binding (by fluorescence intensity and polarization) |
Day 3 (June 10th)
| Lectures: | 9:00 - 10:30 | Fluorescence Microscopy + FCS |
| Lab: | 10:30 - 14:00 | Introduction to two-photon microscope, antibody binding probed by FCS, diffusion of green fluorescent protein in living cells |
We will take short breaks between lectures. Note that beverages and food are not included in the workshop. Please bring your own coffee and snacks.