University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, October 19th 2017
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Jose Alejo Amaya, Engelhart/Adamala lab, GCD, University of Minnesota
Subject: The Unexplored Mechanisms and Regularatory Functions of Ribsome Translocation

In every cell, protein synthesis is carried out by the the ribosome, a complex macromolecular RNA-protein assembly. Single-molecule fluorescence methods have been applied to decipher the mechanism and regulatory roles of the multi-step process of translocation that accompanies every round of translation. The elongation factor G(EF-G)-catalyzed movement of substrates through the ribosome was examined from several perspectives or signals reporting on various ribosomal degrees of freedom. Two states interconvert in the presence of EF-G(GDP), displaying novel head domain motions, until translocation is completed. In order to test if the mentioned state fluctuations leading to translocation are correlated to the engagement of the P-site by the incoming peptidyl-tRNA, the translocation of miscoded tRNAs was studied. Severe defects in the late stages of translocation reveal the correlation between this new stage of translocation and P-site tRNA engagement.

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