University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, December 7th 2017
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Renee Frontiera, University of Minnesota
Subject: Towards label-free super-resolution imaging

Super-resolution imaging methods have revolutionized our understanding of biological processes on nanometer length scales, due to their ability to break the optical diffraction limit. These new techniques enable direct visualization of fluorescently-labeled samples on the 10 nm length scale. However, the fluorescent labels required by current super-resolution imaging approaches can be perturbation to structure and function, and fluorophores are prone to significant photobleaching. Thus, we have developed a novel super-resolution imaging technique which does not require the use of fluorescent labels, based on chemically-specific Raman imaging. We combine stimulated Raman microscopy with concepts from stimulated emission depletion microscopy to achieve label-free sub-diffraction imaging. This talk will cover the photophysical principles behind super-resolution Raman imaging, highlight the highest resolution achieved to date, and discuss biological applications of the technique, particularly with respect to imaging nanoscale cellular membrane dynamics.

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