University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, March 30th 2017
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Prof. Peter Kner, University of Georgia
Subject: Wavefront Correction for Superresolution Microscopy

Superresolution microscopy is rapidly becoming an essential tool in the biological sciences allowing imaging biological structure at length scales below 250 nm. Currently, superresolution microscopy has been applied successfully on single cells achieving resolutions of 100nm down to 20nm over a few microns of depth. When superresolution microscopy is applied in thicker samples the resolution rapidly degrades. Optical aberrations and scattering distort and reduce the point spread function causing different superresolution techniques to fail in different ways. I will discuss our work on combining structured illumination microscopy and stochastic optical reconstruction microscopy with adaptive optics to achieve sub-diffraction resolution in thick tissue.

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