University of Minnesota
School of Physics & Astronomy

Physics and Astronomy Calendar

Tuesday, July 22nd 2014
10:00 am:
Academic Calendar in NHH 2-101 
Speaker: Jesse McCaffrey, Biochem, Molec Biol/Biophysics, University of Minnesota
Subject: Elucidating the Structural Dynamics of SERCA-PLB Regulation by Electron Paramagnetic Resonance
This is the Public Portion of Mr. McCaffrey's thesis defense

In muscle cells, contraction and relaxation are regulated by intracellular calcium, which is transported by the SR calcium ATPase (SERCA). In cardiac muscle cells, SERCA activity is inhibited by binding of phospholamban (PLB), though this inhibition is relieved by phosphorylation of PLB during adrenaline response. The mechanisms for this inhibition and relief of inhibition are unknown, so I use electron paramagnetic resonance (EPR) in conjunction with site-directed spin-labeling to study the structural dynamics of SERCA, PLB, and the SERCA-PLB complex. I will present results on SERCA and PLB homo/hetero-association, as well as orientation studies that help elucidate the SERCA-PLB interaction as affected by phosphorylation.

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