University of Minnesota
School of Physics & Astronomy

Physics and Astronomy Calendar

Thursday, April 25th 2019
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Siddarth Karuka, PhD student in Jochen Mueller’s lab, School of Physics and Astronomy
Subject:  Progress Report: Axial Super-Resolution with Two-Photon Microscopy

The Nuclear Envelope (NE) is a ~40 nm space enclosed by the Outer and Inner Nuclear Membrane (ONM and INM), that separates the nucleus from the cytoplasm. Although recent research has identified the NE as a critical signaling hub for a cell, it remains difficult to study with current fluorescence microscopy techniques that are limited to 50 nm axial resolution. To study systems like these, we have developed the dual color z-scan (DC Z-Scan) technique that can achieve axial resolution on the order of a nanometer. We first demonstrate the technique on a supported lipid bilayer, and then use it to measure the thickness of NE, distinguish proteins that reside on the ONM vs INM and study the translocation kinetics of these proteins.

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