University of Minnesota
School of Physics & Astronomy

Physics and Astronomy Calendar

Thursday, October 31st 2019
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Jacob Ritz, UMN, Puchner Lab
Subject: Progress Report - Development of diffusion contrast Photo-Activated Localization Microscopy (dcPALM) to study sparse and transient protein interactions in live cells.

Cells make decisions based on signaling cascades, which involve interactions of many unique molecules. Measuring the properties of these interactions is not only key to understanding how cells make decisions, but this information can also be used to rectify pathological dysfunction of these pathways. Some signaling molecules are present in concentrations 1000-fold higher than that of their binding partners, making interactions between partners hard to observe, especially in live cells. Here I propose a PALM imaging technique that reduces the background signal from non-interacting molecules by exploiting their faster diffusion compared to the interacting species. The apparent reduction of non-interacting molecules could reveal dynamic interactions that have yet to be observed, as well as allowing me to characterize binding kinetics of known interactions. I will show some preliminary data in a development system show a proof of concept, and also touch on a few systems of interest I plan to study with this technique.

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