University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, April 19th 2018
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Ashim Rai, (postdoc in Shiv’s lab) Department of Genetics, Cell Biology and Development, University of Minnesota
Subject: Biophysical regulation of myosin VI motility by cargo adaptor proteins

: Cargo transport by motor proteins organizes the cell interior. On cellular cargoes, the binding of motor proteins is mediated by cargo adaptor proteins. Initially thought of as passive scaffolds for motor proteins, cargo adaptor proteins have recently been shown to directly affect motor function through structural studies. However, a biophysical mechanism of cargo adaptor-mediated regulation of motor activity is still lacking. In this study, we have tried to address this problem in the context of the minus-end directed actin motor, Myosin VI. Through direct measurements of adapter-mediated changes in myosin VI motility, conformation and dimerization, we have tried to establish a structure-function relationship between myosin VI and its cargo adaptor proteins. We find that binding to cargo adaptor has a potentiating effect on myosin VI velocity and processivity which is mediated through a combination of auto inhibition release, lever arm extension and dimerization of the myosin VI motor.

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