University of Minnesota
School of Physics & Astronomy

Biophysics Seminar

Thursday, April 25th 2019
10:10 am:
Biophysics Seminar in 120 PAN
Speaker: Siddarth Karuka, PhD student in Jochen Mueller’s lab, School of Physics and Astronomy
Subject:  Progress Report: Axial Super-Resolution with Two-Photon Microscopy

The Nuclear Envelope (NE) is a ~40 nm space enclosed by the Outer and Inner Nuclear Membrane (ONM and INM), that separates the nucleus from the cytoplasm. Although recent research has identified the NE as a critical signaling hub for a cell, it remains difficult to study with current fluorescence microscopy techniques that are limited to 50 nm axial resolution. To study systems like these, we have developed the dual color z-scan (DC Z-Scan) technique that can achieve axial resolution on the order of a nanometer. We first demonstrate the technique on a supported lipid bilayer, and then use it to measure the thickness of NE, distinguish proteins that reside on the ONM vs INM and study the translocation kinetics of these proteins.

The weekly calendar is also available via subscription to the physics-announce mailing list, and by RSS feed.